Fsc-a

Here, "FSC-A" is calculated from the image mask. While less common, the same principle applies: area vs. height (or aspect ratio) weeds out doublets and clusters. However, imaging provides the ultimate confirmation – you can literally see if it’s a doublet.

Depending on your field, "FSC-A" might occasionally refer to: Here, "FSC-A" is calculated from the image mask

A singlet cell passing through a uniform laser beam produces a pulse where the Area is directly proportional to the Height. Here, "FSC-A" is calculated from the image mask